Sanger sequencing data

The Sanger importer is designed to import large volumes of Sanger high-throughput sequencing data. Formats supported are ab, abi, ab1, scf and phd. Uncompressed files as well as files compressed using gzip (.gz), zip (.zip) or bzip2 (.bz2) can be provided as input.

To launch the Sanger importer, go to:

        Import (Image Next_Folder_16_n_p) | Sanger (Image color_ngs_import_16_n_p).

This opens a dialog where files can be selected and import options specified (figure 7.21).

Image importngsdialog-sanger
Figure 7.21: Importing Sanger sequencing data.

Sanger sequencing data can also be imported using the Standard Import (Image Next_Folder_16_n_p) (see Standard import). The following are key differences of the high-throughput importer when compared to the Standard Import:

Sanger data can also be imported using the on-the-fly import functionality available in workflows, described in Launching workflows individually and in batches. Both the Sanger importer and the Standard Import ("Trace files") are available using the on-the-fly import.

The General options to the left are:

The next wizard step provides some options for handling the results. When the option to "Create subfolders per batch unit" is enabled, each sequence list created is put into its own subfolder. This can be helpful for running analyses in batches and for organizing the results of subsequent analyses.