RNA-Seq analysis, expression analysis, and other tools can be included in workflows. Designing a workflow that includes an RNA-Seq Analysis step, which is typically run once per sample, and an expression analysis step, typically run once to analyze all the samples, is described in Batching part of a workflow.
The statistical analysis and visualization tools of the RNA-Seq folder make extensive use of the metadata system. For example, metadata are required when defining the experimental design in the Differential Expression for RNA-Seq tool, and can be used to add extra layers of insight in the Create Heat Map and PCA for RNA-Seq tools.
To get the most out of these tools we recommend that all input expression tracks are associated with metadata. Importing metadata and associated data elements with it is described in Metadata.
- RNA-Seq normalization
- Create Expression Browser
- miRNA analysis
- RNA-Seq Tools
- Expression Plots
- Differential Expression