When both forward and reverse regions are defined

If both a forward and a reverse region are defined, primer pairs will be suggested by the algorithm.

After pressing the Calculate button a dialog will appear (see figure 17.8).

Image pcrcalculationdialog
Figure 17.8: Calculation dialog for PCR primers when two primer regions have been defined.

Again, the top part of this dialog shows the parameter settings chosen in the Primer parameters preference group which will be used by the design algorithm. The lower part again contains a menu where the user can choose to include mispriming of both primers as a criteria in the design process (see When a single primer region is defined). The central part of the dialog contains parameters pertaining to primer pairs. Here three parameters can be set: