This tool is designed to report the performance (enrichment and
specificity) of a targeted re-sequencing experiment.
Targeted re-sequencing is due to its low costs, very popular and several
companies provide platforms and protocols (learn more at
). Array-based approaches are offered by e.g. Agilent (SureSelect) and Roche Nimblegen. Furthermore, amplicon sequencing with PCR primers is offered by RainDance, Fluidigm and others.
Given an annotation track with the target regions (e.g. imported from a bed
file), this tool will investigate a read mapping to determine whether the
targeted regions have been appropriately covered by sequencing reads as well as
information about how specific the reads map to the targeted regions. The
results are provided both as a summary report and as track or table with
detailed information about each targeted region.