Number of cut sites

Clicking Next confirms the list of enzymes which will be included in the analysis, and takes you to the dialog shown in figure 19.43.

Image restrictionstep3_web
Figure 19.43: Selecting number of cut sites.

If you wish the output of the restriction map analysis only to include restriction enzymes which cut the sequence a specific number of times, use the checkboxes in this dialog:

The default setting is to include the enzymes which cut the sequence one or two times.

You can use the checkboxes to perform very specific searches for restriction sites: e.g. if you wish to find enzymes which do not cut the sequence, or enzymes cutting exactly twice.