Sanger sequencing data

Although traditional sequencing data (with chromatogram traces like abi files) is usually imported using the standard Import (Image import), see Import bioinformatics data, this option has also been included in the High-Throughput Sequencing Data import. It is designed to handle import of large amounts of sequences, and there are three differences from the standard import: With the standard import, it is practically impossible to import up to thousands of trace files and use them in an assembly. With this special High-Throughput Sequencing import, there is no limit. The import formats supported are the same: ab, abi, ab1, scf and phd.

For all formats, compressed data in gzip format is also supported (.gz).

The dialog for importing data Sanger sequencing data is shown in figure 6.10.

Image importngsdialog-sanger
Figure 6.10: Importing data from Sanger sequencing.

The General options to the left are:

Click Next to adjust how to handle the results. We recommend choosing Save in order to save the results directly to a folder, since you probably want to save anyway before proceeding with your analysis. There is an option to put the import data into a separate folder. This can be handy for better organizing subsequent analysis results and for batch processing.