Molecular cloning

Molecular cloning is a very important tool in the quest to understand gene function and regulation. Through molecular cloning it is possible to study individual genes in a controlled environment. Using molecular cloning it is possible to build complete libraries of fragments of DNA inserted into appropriate cloning vectors.

The in silico cloning process in CLC Genomics Workbench begins with the selection of sequences to be used:

        Toolbox | Molecular Biology Tools (Image lab_work_support) | Cloning and Restriction Sites (Image cloningandrestrictionsites)| Cloning (Image cloning)

This will open a dialog where you can select the sequences containing the fragments you want to clone (figure 19.1).

Image cloningwizardstep1
Figure 19.1: Selecting one or more sequences containing the fragments you want to clone.

Note that the vector sequence will be selected when you click Next as shown in figure figure 19.2.

Image cloningwizardstep2
Figure 19.2: Selecting a cloning vector.

Select the cloning vector by clicking the browse (Image browse) button. Once the sequence has been selected, click Finish. The CLC Genomics Workbench will now create a sequence list of the fragments and vector sequences and open it in the cloning editor as shown in figure 19.3.

Image cloningviewinitial
Figure 19.3: Cloning editor.

When you save the cloning experiment, it is saved as a Sequence list. See Sequence lists for more information about sequence lists. If you need to open the list later for cloning work, simply switch to the Cloning (Image cloning) editor at the bottom of the view.

If you later in the process need additional sequences, you can easily add more sequences to the view. Just:

        right-click anywhere on the empty white area | Add Sequences