• Product manuals

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• Introduction
  • The concept of QIAGEN CLC Microbial Genomics Module
  • Contact information
  • System requirements
  • Installing modules
    • Licensing modules
    • Uninstalling modules
  • Installing server extensions
    • Licensing server extensions
• Microbial template workflows
  • Taxonomic Analysis template workflows
    • Data QC and Remove Background Reads
    • Data QC and Taxonomic Profiling
    • Merge and Estimate Alpha and Beta diversities
    • QC, Assemble and Bin Pangenomes
  • Amplicon-Based Analysis template workflows
    • Data QC and OTU Clustering
    • Detect Amplicon Sequence Variants and Assign Taxonomies
    • Estimate Alpha and Beta Diversities
  • Typing and Epidemiology template workflows
    • Compare Variants Across Samples
    • Create MLST Scheme with Sequence Types
    • Map to Specified Reference
    • Type Among Multiple Species
    • Type a Known Species
    • Type Influenza Strain
  • QIAseq Analysis template workflows
    • Analyze QIAseq xHYB Mycobacterium tuberculosis and NTM-ID Panel Data (Human host)
    • Analyze QIAseq xHYB Viral Panel Data (Human host)
    • Find QIAseq xHYB AMR Markers (Human host)
  • QIAseq Panel Analysis Assistant
• De Novo Assemble Small Genome
  • De Novo Assemble Small Genome parameters
  • De Novo Assemble Small Genomes output
• De Novo Assemble Metagenome
  • De Novo Assemble Metagenome parameters
  • De Novo Assemble Metagenome output
• Amplicon-Based Analysis
  • Normalize OTU Table by Copy Number
  • Filter Samples Based on Number of Reads
  • OTU clustering
    • OTU clustering parameters
    • OTU clustering outputs
    • Importing and exporting OTU abundance tables
  • Align OTUs using MUSCLE
  • Detect Amplicon Sequence Variants
    • Detect Amplicon Sequence Variants parameters
    • Detect Amplicon Sequence Variants output
    • Importing ASV abundance tables
  • Classify Long Read Amplicons
    • Classify Long Read Amplicons parameters
    • Classify Long Read Amplicons output
• Taxonomic Analysis
  • Contig Binning
    • Bin Pangenomes by Taxonomy
    • The Taxonomy Binning Report
    • Bin Pangenomes by Sequence
  • Identify Viral Integration Sites
    • The Viral Integration Viewer
    • The Viral Integration Report
  • Classify Whole Metagenome Data
    • Classify Whole Metagenome Data parameters
    • Classify Whole Metagenome Data output
    • Classify Whole Metagenome Data abundance table
  • Taxonomic Profiling
    • Taxonomic Profiling parameters
    • Taxonomic Profiling output
    • Taxonomic Profiling abundance table
• Abundance Analysis
  • Merge Abundance Tables
    • Merge Abundance Tables output
  • Refine Abundance Table
    • Refine Abundance Table parameters
    • Refine Abundance Table output
  • Assign Taxonomies to Sequences in Abundance Table
  • Alpha Diversity
    • Alpha diversity measures
  • Beta Diversity
    • Beta diversity measures
  • PERMANOVA Analysis
  • Differential Abundance Analysis
  • Create Heat Map for Abundance Table
    • Clustering of features and samples
    • The heat map view
    • Create heat map for specific taxonomic level
  • Add Metadata to Abundance Table
• Find the best matching reference
  • Find Best Matches using K-mer Spectra
    • From samples best matches to a common reference for all
  • Find Best References using Read Mapping
    • Output from Find Best References using Read Mapping
    • High-sensitivity mapping
  • Type with Consensus Refinement
    • Type with Consensus Refinement parameters
    • Type with Consensus Refinement outputs
    • Type with Consensus Refinement report
• Phylogenetic trees using SNPs or k-mers
  • Create SNP Tree
    • SNP tree report
    • SNP tree
    • SNP Matrix
  • Create K-mer Tree
    • Visualization of K-mer Tree for identification of common reference
• MLST Typing Tools
  • Getting started with the MLST Typing tools
  • MLST Scheme Visualization and Management
  • Minimum Spanning Trees
    • The Minimum Spanning Tree view
    • Navigating the Tree view
    • The Minimum Spanning Tree Side Panel
  • Identify MLST Scheme from Genomes
  • Type with MLST Scheme
    • Type with MLST Scheme output
  • Compare MLST Typing Results
    • Compare MLST Typing Results output
  • Add Typing Results to MLST Scheme
• Additional Typing Tools
  • Spoligotype Mycobacterium Tuberculosis
    • Spoligotype Mycobacterium Tuberculosis parameters
    • Spoligotype Mycobacterium Tuberculosis output
• Functional Analysis
  • Find Prokaryotic Genes
  • Annotate with BLAST
  • Annotate with DIAMOND
  • Annotate CDS with Best BLAST Hit
  • Annotate CDS with Best DIAMOND Hit
  • Annotate CDS with Pfam Domains
  • Build Functional Profile
    • Functional profile abundance table
  • Infer Functional Profile
  • Identify Pathways
    • Called Pathways Result
    • The Identified Pathways View
• Drug Resistance Analysis
  • Find Resistance with PointFinder
  • Find Resistance with Nucleotide Database
  • Find Resistance with ShortBRED
    • Resistance abundance table
  • Join Nearby Variants
• Databases for MLST Schemes
  • Create MLST Scheme
  • Download MLST Scheme
    • Download MLST Scheme parameters
  • Import MLST Scheme
• Databases for Amplicon-Based Analysis
  • Download Amplicon-Based Reference Database
• Databases for Taxonomic Analysis
  • Download Curated Microbial Reference Database
  • Download Custom Microbial Reference Database
    • Database Builder
  • Download Pathogen Reference Database
  • Create Whole Metagenome Index
  • Create Taxonomic Profiling Index
• Databases for Functional Analysis
  • Download Protein Database
  • Download Ontology Database
    • The GO Database View
    • The EC Database View
  • Download Pathway Database
    • The Pathway Database
    • The Pathway View
  • Create DIAMOND Index
  • Import RNAcentral Database
  • Import PICRUSt2 Multiplication Table
• Databases for Drug Resistance Analysis
  • Download Resistance Database
  • Reference Data Elements
• QIAseq 16S/ITS Demultiplexer
• Utility Tools
  • Mask Low-Complexity Regions
    • Mask Low-Complexity Regions Report
  • Result Metadata
    • Create a Result Metadata Table
    • Running an analysis directly from a Result Metadata Table
    • Extend Result Metadata Table
    • Use Genome as Result
• Legacy workflows
  • Analyze QIAseq xHYB Mycobacterium Tuberculosis Panel Data (Human host)
  • Analyze QIAseq xHYB NTM-ID Panel Data (Human host)
• Legacy CLC objects
  • Legacy MLST Typing Result
• Appendix
  • Using the Assembly ID attribute
• Bibliography

Using the Assembly ID attribute

The Assembly ID attribute on sequences is used by many tools of the QIAGEN CLC Microbial Genomics Module to group sequences into meaningful entities, e.g. to group all contigs of a draft assembly. In order to see how these tools utilize the "Assembly ID" attribute, please read the tool manual.

Tools that are aware of this attribute include:

•  Bin Pangenomes by Sequence
•  Create K-mer Tree
•  Create MLST Scheme
•  Create Taxonomic Profiling Index
•  Find Best Matches using K-mer Spectra
•  Find Prokaryotic Genes

Attributes can be added to sequences in a sequence list in the following way:

1. Open the Table view of a sequence list.
2. Select all rows corresponding to sequences that should be grouped.
3. Right-click on the selection and choose Add Attributes....
4. Select Assembly ID from the dropdown menu in the Name field.
5. Enter a string in the Value field to uniquely identify the assembly.

For large sequence lists containing many assemblies it may be beneficial to use Update Sequence Attributes instead.

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