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• Introduction
  • The concept of CLC Microbial Genomics Module
  • Contact information
  • System requirements
  • Installing modules
    • Licensing modules
    • Uninstalling modules
  • Installing server extensions
    • Licensing server extensions
• Microbial template workflows
  • Taxonomic Analysis template workflows
    • Data QC and Remove Background Reads
    • Data QC and Taxonomic Profiling
    • Merge and Estimate Alpha and Beta diversities
    • QC, Assemble and Bin Pangenomes
  • Amplicon-Based Analysis template workflows
    • Data QC and OTU Clustering
    • Detect Amplicon Sequence Variants and Assign Taxonomies
    • Estimate Alpha and Beta Diversities
  • Typing and Epidemiology template workflows
    • Compare Variants Across Samples
    • Create MLST Scheme with Sequence Types
    • Map to Specified Reference
    • Type Among Multiple Species
    • Type a Known Species
  • QIAseq Analysis template workflows
    • Analyze QIAseq xHYB Mycobacterium Tuberculosis Panel Data (Human host)
    • Analyze QIAseq xHYB Viral Panel Data (Human host)
    • Find QIAseq xHYB AMR Markers (Human host)
  • QIAseq Panel Analysis Assistant
• De Novo Assemble Small Genome
  • De Novo Assemble Small Genome parameters
  • De Novo Assemble Small Genomes output
• De Novo Assemble Metagenome
  • De Novo Assemble Metagenome parameters
  • De Novo Assemble Metagenome output
• Amplicon-Based Analysis
  • Normalize OTU Table by Copy Number
  • Filter Samples Based on Number of Reads
  • OTU clustering
    • OTU clustering parameters
    • OTU clustering outputs
    • Importing and exporting OTU abundance tables
  • Align OTUs with MUSCLE
  • Detect Amplicon Sequence Variants
    • Detect Amplicon Sequence Variants parameters
    • Detect Amplicon Sequence Variants output
    • Importing ASV abundance tables
  • Classify Long Read Amplicons
    • Classify Long Read Amplicons parameters
    • Classify Long Read Amplicons output
• Taxonomic Analysis
  • Contig Binning
    • Bin Pangenomes by Taxonomy
    • The Taxonomy Binning Report
    • Bin Pangenomes by Sequence
  • Identify Viral Integration Sites
    • The Viral Integration Viewer
    • The Viral Integration Report
  • Classify Whole Metagenome Data
    • Classify Whole Metagenome Data parameters
    • Classify Whole Metagenome Data output
    • Classify Whole Metagenome Data abundance table
  • Taxonomic Profiling
    • Taxonomic Profiling parameters
    • Taxonomic Profiling output
    • Taxonomic Profiling abundance table
• Abundance Analysis
  • Merge Abundance Tables
    • Merge Abundance Tables output
  • Refine Abundance Table
    • Refine Abundance Table parameters
    • Refine Abundance Table output
  • Assign Taxonomies to Sequences in Abundance Table
  • Alpha Diversity
    • Alpha diversity measures
  • Beta Diversity
    • Beta diversity measures
  • PERMANOVA Analysis
  • Differential Abundance Analysis
  • Create Heat Map for Abundance Table
    • Clustering of features and samples
    • The heat map view
    • Create heat map for specific taxonomic level
  • Add Metadata to Abundance Table
• Find the best matching reference
  • Find Best Matches using K-mer Spectra
    • From samples best matches to a common reference for all
  • Find Best References using Read Mapping
    • The Find Best References using Read Mapping Report
• Phylogenetic trees using SNPs or k-mers
  • Create SNP Tree
    • SNP tree report
    • SNP tree
    • SNP Matrix
  • Create K-mer Tree
    • Visualization of K-mer Tree for identification of common reference
• MLST Scheme Tools
  • Getting started with the MLST Scheme tools
  • MLST Scheme Visualization and Management
  • Minimum Spanning Trees
    • The Minimum Spanning Tree view
    • Navigating the Tree view
    • The Layout panel
    • The Metadata panel
  • Type With MLST Scheme
    • Type With MLST Scheme results
    • The MLST Typing Result element
  • Add Typing Results to MLST Scheme
  • Identify MLST Scheme from Genomes
• Additional Typing Tools
  • Spoligotype Mycobacterium Tuberculosis
    • Spoligotype Mycobacterium Tuberculosis parameters
    • Spoligotype Mycobacterium Tuberculosis output
• Functional Analysis
  • Find Prokaryotic Genes
  • Annotate with BLAST
  • Annotate with DIAMOND
  • Annotate CDS with Best BLAST Hit
  • Annotate CDS with Best DIAMOND Hit
  • Annotate CDS with Pfam Domains
  • Build Functional Profile
    • Functional profile abundance table
  • Infer Functional Profile
  • Identify Pathways
    • Called Pathways Result
    • The Identified Pathways View
• Drug Resistance Analysis
  • Find Resistance with PointFinder
  • Find Resistance with Nucleotide Database
  • Find Resistance with ShortBRED
    • Resistance abundance table
  • Join Nearby Variants
• Databases for MLST Schemes
  • Create MLST Scheme
  • Download MLST Scheme
    • Download MLST Scheme parameters
  • Import MLST Scheme
• Databases for Amplicon-Based Analysis
  • Download Amplicon-Based Reference Database
• Databases for Taxonomic Analysis
  • Download Curated Microbial Reference Database
  • Download Custom Microbial Reference Database
    • Database Builder
  • Download Pathogen Reference Database
  • Create Whole Metagenome Index
  • Create Taxonomic Profiling Index
• Databases for Functional Analysis
  • Download Protein Database
  • Download Ontology Database
    • The GO Database View
    • The EC Database View
  • Download Pathway Database
    • The Pathway Database
    • The Pathway View
  • Create DIAMOND Index
  • Import RNAcentral Database
  • Import PICRUSt2 Multiplication Table
• Databases for Drug Resistance Analysis
  • Download Resistance Database
  • Reference Data Elements
• QIAseq 16S/ITS Demultiplexer
• Utility Tools
  • Mask Low-Complexity Regions
    • Mask Low-Complexity Regions Report
  • Result Metadata
    • Create a Result Metadata Table
    • Running an analysis directly from a Result Metadata Table
    • Extend Result Metadata Table
    • Use Genome as Result
• Legacy tools
  • Remove OTUs with Low Abundance
• Appendix
  • Using the Assembly ID annotation
• Bibliography

QIAseq Analysis template workflows

The QIAseq Analysis template workflows are found at:

        Workflows | Template Workflows () | Microbial Workflows () | QIAseq Analysis ()

The QIAseq template workflows are configured for selection of Reference Data Sets, making them simple to launch while helping to ensure that the same reference data is used consistently. QIAGEN Reference Sets are available for download using the Reference Data Manager in the CLC Genomics Workbench. Reference data for a specific workflow can also be downloaded via the workflow launch wizard.

For further information about how to work with reference data when launching a workflow, and how to configure workflows to support use of Reference Data Sets, see https://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Reference_data_workflows.html.
For further information about QIAGEN Sets, see https://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=QIAGEN_Sets.html.

Launching the QIAseq Analysis template workflows

The following sections describe how to launch the QIAseq Analysis template workflows from the Workflows menu. They can also be launched from the QIAseq Panel Analysis Assistant, where they can be found in the xHYB Viral and Bacterial category (see QIAseq Panel Analysis Assistant).

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Subsections

• Analyze QIAseq xHYB Mycobacterium Tuberculosis Panel Data (Human host)
• Analyze QIAseq xHYB Viral Panel Data (Human host)
• Find QIAseq xHYB AMR Markers (Human host)

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