Classify Long Read Amplicons parameters

To run the tool, go to:

        Toolbox | Template Workflows (Image workflow_group) | Microbial Workflows (Image mgm_folder_closed_flat_16_h_p) | Metagenomics (Image wma_folder_open_flat_16_n_p) | Amplicon-Based Analysis (Image otutools_open_16_n_p) | Classify Long Read Amplicons (Image classify_lr_amplicon_16_n_p)

Classify Long Read Amplicons takes single-end long-read amplicon reads as input. The data should be trimmed for adapters, barcodes, and preferably also primers. Quality scores are not needed for the tool to work and you do not need to quality trim the reads. Since the algorithm infers an error model based on the read alignments, samples from different runs should not be analyzed simultaneously. Instead, you can run samples in "Batch" mode.

In the wizard, three categories of parameter settings need to be set (figure 4.21):

Image createamplicon_setting
Figure 4.21: Classify Long Read Amplicons parameter settings.