Detect Amplicon Sequence Variants parameters

To run the Detect Amplicon Sequence Variants tool, go to

        Toolbox | Microbial Genomics Module (Image mgm_folder_closed_flat_16_h_p) | Metagenomics (Image wma_folder_open_flat_16_n_p) | Amplicon-Based Analysis (Image otutools_open_16_n_p) | Detect Amplicon Sequence Variants (Image detect_amplicon_sequence_variants_16_n_p)

Select the single- or paired-end sequence lists to be analyzed. For paired reads, pairs should have a minimum overlap of 12 bases. Data should be trimmed beforehand to remove adapters and poor quality nucleotides. This can be done using Trim Reads (

Set the Trim and filter parameters (figure 4.13):

Image detect_asv_trim_filter
Figure 4.13: Trim and filter parameter settings