Download Amplicon-Based Reference Database

Amplicon-based reference databases contain a list of representative amplicon sequences and their taxonomy. Databases like these are required for amplicon-based analysis (Amplicon-Based Analysis).

The following databases are available:

Some of the above databases are available at different clustering levels of sequence similarities.

To run the tool, go to

        Toolbox | Microbial Genomics Module (Image mgm_folder_closed_flat_16_h_p) | Metagenomics (Image wma_folder_open_flat_16_n_p) | Databases (Image databases_folder_closed_16_n_p) | Amplicon-Based Analysis (Image otutools_open_16_n_p) | Download Amplicon-Based Reference Database (Image download_otu_refdb_16_n_p)

Select the database needed and specify where to save it. When using this tool, the databases downloaded are automatically formatted.

If you wish to format your own database with your own sequences and a corresponding taxonomy file, use the Update Sequence Attributes in Lists tool (https://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Update_Sequence_Attributes_in_Lists.html) to set the "Taxonomy" field. A clustering level for such custom databases can not be set on the data object directly, but it may be specified as a parameter when running the OTU Clustering tool.