Detect Amplicon Sequence Variants

The Detect Amplicon Sequence Variants tool infers sequence variants from amplicon data. The tool uses error profiling to distinguish biological nucleotide differences from sequencing errors, making it possible to resolve amplicon sequence variants (ASVs) down to the level of single nucleotide differences. The algorithm is inspired by DADA2, [Callahan et al., 2016].

The Detect Amplicon Sequence Variants analysis includes the following steps:

A template workflow with a proposed analysis pipeline - trimming reads, detecting amplicon sequence variants, merging ASV tables, and assigning taxonomies - is available at:

        Toolbox | Template Workflows (Image workflow_group) | Microbial Workflows (Image mgm_folder_closed_flat_16_h_p) | Metagenomics (Image wma_folder_open_flat_16_n_p) | Amplicon-Based Analysis (Image otutools_open_16_n_p) | Detect Amplicon Sequence Variants and Assign Taxonomies workflow (Image detect_amplicon_sequence_variants_16_n_p)

For more information, see Detect Amplicon Sequence Variants and Assign Taxonomies workflow.



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