Sanger sequencing data

Although traditional sequencing data (with chromatogram traces like abi files) is usually imported using the standard Import (Image Next_Folder_16_n_p), see Import bioinformatics data, this option has also been included in the High-Throughput Sequencing Data import. It is designed to handle import of large amounts of sequences, and there are three differences from the standard import: With the standard import, it is practically impossible to import up to thousands of trace files and use them in an assembly. With this special High-Throughput Sequencing import, there is no limit. The import formats supported are the same: ab, abi, ab1, scf and phd.

For all formats, compressed data in gzip format is also supported (.gz).

The dialog for importing data Sanger sequencing data is shown in figure 6.11.

Image importngsdialog-sanger
Figure 6.11: Importing data from Sanger sequencing.

The General options to the left are:

Click Next to adjust how to handle the results. We recommend choosing Save in order to save the results directly to a folder, since you probably want to save anyway before proceeding with your analysis. There is an option to put the import data into a separate folder. This can be handy for better organizing subsequent analysis results and for batch processing.