Separate fragments of sequences on gel
This section explains how to simulate a gel electrophoresis of one or more sequences which are digested with restriction enzymes. There are two ways to do this:
- When performing the Restriction Site Analysis from the Toolbox, you can choose to create a restriction map which can be shown as a gel. This is explained in Restriction sites output options.
-
From all the graphical views of sequences, you can
right-click the name of the sequence and choose: Digest Sequence with Selected Enzymes and Run on Gel (
). The views
where this option is available are listed below:
- Circular view (see Circular view view).
- Ordinary sequence view (see View sequence).
- Graphical view of sequence lists (see Sequence lists). Cloning editor (see Cloning editor). Primer designer (see Primer designer).
Note! When using the right-click options, the sequence will be digested with the enzymes that are selected in the Side Panel. This is explained in Restriction Sites .
The view of the gel is explained here