Nested structure elements
The structure elements involving nested base pairs can be classified by a given base pair and the other base pairs that are nested and accessible from this pair. For a more elaborate description we refer the reader to [Sankoff et al., 1983] and [Zuker and Sankoff, 1984].If the nucleotides with position number form a base pair and , then we say that the base pair is accessible from if there is no intermediate base pair such that . This means that is nested within the pair and there is no other base pair in between.
Using the number of accessible pase pairs, we can define the following distinct structure elements:
- Hairpin loop (). A base pair with 0 other accessible base pairs forms a hairpin loop. The energy contribution of a hairpin is determined by the length of the unpaired (loop) region and the two bases adjacent to the closing base pair which is termed a terminal mismatch (see figure 22.29A).
- A base pair with 1 accessible base pair can give rise to three distinct structure elements:
- Stacking of base pairs (). A stacking of two consecutive pairs occur if . Only canonical base pairs ( or or ) are allowed (see figure 22.29B). The energy contribution is determined by the type and order of the two base pairs.
- Bulge (). A bulge loop occurs if or but not both. This means that the two base pairs enclose an unpaired region of length 0 on one side and an unpaired region of length on the other side (see figure 22.29C). The energy contribution of a bulge is determined by the length of the unpaired (loop) region and the two closing base pairs.
- Interior loop ().An interior loop occurs if both and This means that the two base pairs enclose an unpaired region of length on both sides (see figure 22.29D). The energy contribution of an interior loop is determined by the length of the unpaired (loop) region and the four unpaired bases adjacent to the opening- and the closing base pair.
- Multi loop opened (). A base pair with more than two accessible base pairs gives rise to a multi loop, a loop from which three or more stems are opened (see figure 22.29E). The energy contribution of a multi loop depends on the number of Stems opened in multi-loop () that protrude from the loop.