Creating your own miRBase file
If you wish to construct a file yourself to be used as a miRBase file for annotation, this is also possible if you format the file in the same way as the miRBase data file. In particular, the following needs to be in place:- The sequences needs "miRNA" annotation on the precursor sequences. In the Workbench, you can add a miRNA annotation by selecting a region and right click to Add Annotation. You should have a max 2 miRNA annotations per precursor sequences. Matches to first miRNA annotation are counting in
5'column. Matches to second miRNA annotation are counted as3'matches. - If you have sequence list containing sequences form multiple species, the Latin name of the sequences should be set. This is used in the Annotate and merge small RNA samples dialog where you can select the species. If the Latin name is not set, the dialog will show "N/A".