Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer)
The Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) template workflow supports a combined N6-T RT primer + ODT-RT primer protocol. All reads are used for gene expression analysis while the reads amplified by the N6-T RT primer are used for studying transcript expression and fusions.
The Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) workflow can be found here:
Template Workflows | Biomedical Workflows (
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) | Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) (
)
In the first wizard step either select the reads or choose Select files for import to import FASTQ files on-the-fly.
The workflow is designed to analyze all samples within an experiment without the need of batch functionality. It iterates through the samples and processes them separately, and collects and distributes all outputs at the end.
Choose the QIAseq UPXome and FastSelect RNA hg38 reference data when running the workflow standalone.
Keep the "Use organization of input samples" except when you want to add metadata information to the samples. Check that the batch overview looks as expected, especially when using on-the-fly import.
Save the results to a specified location.
Launching using the QIAseq Panel Analysis Assistant
The workflow is also available in the QIAseq Panel Analysis Assistant under FastSelect RNA.
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