Quantify QIAseq UPX 3'
The Quantify QIAseq UPX 3' template workflow can be found here:
Template Workflows | Biomedical Workflows (
) | QIAseq Sample Analysis (
) | QIAseq RNA Workflows (
) | Quantify QIAseq UPX 3' (
)
The Quantify QIAseq UPX 3' workflow takes demultiplexed samples (see Demultiplex QIAseq UPX 3' reads) to be run in batch mode.
The workflow always reports expression across the whole genome, which allows you to see when reads map off-target.
Be aware that the Quantify QIAseq UPX 3' workflow is configured to expect reads that are strand specific. This is because the reaction design ensures that reads from 3' fragments will appear forward-stranded (read 1 mapping sense to the transcript). It is not unusual for 5-10% of reads to be dropped in the workflow for having an unexpected strandedness.
Launching using the QIAseq Panel Analysis Assistant
The workflow is also available in the QIAseq Panel Analysis Assistant under UPX 3' RNA.
Subsections