You can create a local database that you can use for local BLAST searches. You can specify a location on your computer to save the BLAST database files to. The Workbench will list the BLAST databases found in these locations when you set up a BLAST against local data.
DNA, RNA, and protein sequences located in the Navigation Area can be used to create BLAST databases from. Any given BLAST database can only include one molecule type. If you wish to use a pre-formatted BLAST database instead, see Add pre-formatted BLAST databases.
To create a BLAST database, go to:
Toolbox | BLAST ()| Create BLAST Database ()
This opens the dialog seen in figure 13.14.
Select sequences or sequence lists you wish to include in your database and click Next.
In the next dialog, shown in figure 13.15, you provide the following information:
- Name. The name of the BLAST database. This name will be used when running BLAST searches and also as the base file name for the BLAST database files.
- Description. A short description. This is displayed along with the database name in the list of available databases when launching a local BLAST search. If no description is entered, the creation date is used as the description.
- Location. The location to save the BLAST database files to. You can add or change the locations in this list using the Manage BLAST databases dialog.
Create BLAST Database creates BLAST+ version 4 (dbV4) databases.