Manuals
Browse the manual
Introduction to CLC Genomics Workbench
Contact information
System requirements
Limitations on maximum number of cores
Workbench Licenses
Request an evaluation license
Download a license using a license order ID
Import a license from a file
Upgrade license
Configure license server connection
Download a static license on a non-networked machine
Limited mode
About CLC Workbenches
New program feature request
Getting help
CLC Sequence Viewer vs. Workbenches
When the program is installed: Getting started
Quick start
Import of example data
Plugins
Installing plugins
Uninstalling plugins
Updating plugins
Resources
Network configuration
Latest improvements
User interface
View Area
Open view
Show element in another view
Close views
Save changes in a view
Undo/Redo
Arrange views in View Area
Moving a view to a different screen
Side Panel
Zoom and selection in View Area
Zoom in
Zoom out
Selecting, panning and zooming
Toolbox and Status Bar
Processes
Toolbox
Status Bar
Workspace
Create Workspace
Select Workspace
Delete Workspace
List of shortcuts
Data management and search
Navigation Area
Data structure
Create new folders
Sorting folders
Multiselecting elements
Moving and copying elements
Change element names
Delete, restore and remove elements
Show folder elements in a table
Customized attributes on data locations
Configuring which fields should be available
Editing lists
Removing attributes
Changing the order of the attributes
Filling in values
What happens when a clc object is copied to another data location?
Searching
Local search
What kind of information can be searched?
Quick search
Advanced search
Search index
User preferences and settings
General preferences
Default view preferences
Number formatting in tables
Import and export Side Panel settings
Data preferences
Advanced preferences
Default data location
NCBI BLAST
Export/import of preferences
The different options for export and import
View settings for the Side Panel
Saving, removing and applying saved settings
Printing
Selecting which part of the view to print
Page setup
Header and footer
Print preview
Import/export of data and graphics
Standard import
Import using the import dialog
Import using drag and drop
Import using copy/paste of text
External files
Import tracks
Import high-throughput sequencing data
454 from Roche Applied Science
Illumina
SOLiD from Life Technologies
Fasta read files
Sanger sequencing data
Ion Torrent PGM from Life Technologies
Complete Genomics
General notes on handling paired data
SAM and BAM mapping files
Data export
Export of folders and multiple elements in CLC format
Export of dependent elements
Export history
The CLC format
Backing up data from the CLC Workbench
Export of workflow output
Export of tables
Export graphics to files
Which part of the view to export
Save location and file formats
Graphics export parameters
Exporting protein reports
Export graph data points to a file
Copy/paste view output
History log
Element history
Sharing data with history
Batching and result handling
Batch processing
Batch overview
Batch filtering and counting
Setting parameters for batch runs
Running the analysis and organizing the results
How to handle results of analyses
Table outputs
Batch log
Working with tables
Filtering tables
Workflows
Creating a workflow
Adding workflow elements
Configuring workflow elements
Locking and unlocking parameters
Connecting workflow elements
Input and output
Layout
Input modifying tools
Workflow validation
Workflow creation helper tools
Adding to workflows
Snippets in workflows
Supported data flows
Distributing and installing workflows
Creating a workflow installation file
Installing a workflow
Workflow identification and versioning
Automatic update of workflow elements
Executing a workflow
Open copy of installed workflow
Viewing and editing sequences
View sequence
Sequence settings in Side Panel
Restriction sites in the Side Panel
Selecting parts of the sequence
Editing the sequence
Sequence region types
Circular DNA
Using split views to see details of the circular molecule
Mark molecule as circular and specify starting point
Working with annotations
Viewing annotations
Adding annotations
Edit annotations
Removing annotations
Element information
View as text
Sequence Lists
Graphical view of sequence lists
Sequence list table
Extract sequences from sequence list
Data download
GenBank search
GenBank search options
Handling of GenBank search results
Save GenBank search parameters
UniProt (Swiss-Prot/TrEMBL) search
UniProt search options
Handling of UniProt search results
Save UniProt search parameters
Search for structures at NCBI
Structure search options
Handling of NCBI structure search results
Save structure search parameters
Download reference genome data
Selecting data types for download
Cytogenetic ideograms
Sequence web info
Google sequence
NCBI
PubMed References
UniProt
Additional annotation information
BLAST search
Running BLAST searches
BLAST at NCBI
BLAST a partial sequence against NCBI
BLAST against local data
BLAST a partial sequence against a local database
Output from BLAST searches
Graphical overview for each query sequence
Overview BLAST table
BLAST graphics
BLAST table
Extracting a consensus sequence from a BLAST result
Local BLAST databases
Make pre-formatted BLAST databases available
Download NCBI pre-formatted BLAST databases
Create local BLAST databases
Manage BLAST databases
Migrating from a previous version of the Workbench
Bioinformatics explained: BLAST
Examples of BLAST usage
Searching for homology
How does BLAST work?
Which BLAST program should I use?
Which BLAST options should I change?
Explanation of the BLAST output
I want to BLAST against my own sequence database, is this possible?
What you cannot get out of BLAST
Other useful resources
3D Molecule Viewer
Importing molecule structure files
From the Protein Data Bank
From your own file system
BLAST search against the PDB database
Import issues
Viewing molecular structures in 3D
Moving and rotating
Troubleshooting 3D graphics errors
Updating old structure files
Customizing the visualization
Visualization styles and colors
Project settings
Snapshots of the molecule visualization
Tools for linking sequence and structure
Show sequence associated with molecule
Link sequence or sequence alignment to structure
Transfer annotations between sequence and structure
Protein structure alignment
The Align Protein Structure dialog box
Example: alignment of calmodulin
The Align Protein Structure algorithm
General sequence analyses
Extract Annotations
Extract sequences
Shuffle sequence
Dot plots
Create dot plots
View dot plots
Bioinformatics explained: Dot plots
Realization of dot plots
Examples and interpretations of dot plots
Bioinformatics explained: Scoring matrices
Different scoring matrices
Use of scoring matrices
Local complexity plot
Sequence statistics
Bioinformatics explained: Protein statistics
Join sequences
Pattern discovery
Pattern discovery search parameters
Pattern search output
Motif Search
Dynamic motifs
Motif search from the Toolbox
Java regular expressions
Create motif list
Nucleotide analyses
Convert DNA to RNA
Convert RNA to DNA
Reverse complements of sequences
Reverse sequence
Translation of DNA or RNA to protein
Translate part of a nucleotide sequence
Find open reading frames
Open reading frame parameters
Protein analyses
Signal peptide prediction
Signal peptide prediction parameter settings
Signal peptide prediction output
Bioinformatics explained: Prediction of signal peptides
Why the interest in signal peptides?
Different types of signal peptides
Prediction of signal peptides and subcellular localization
The SignalP method
What do the SignalP scores mean?
Protein charge
Modifying the layout
Transmembrane helix prediction
Antigenicity
Plot of antigenicity
Antigenicity graphs along sequence
Hydrophobicity
Hydrophobicity plot
Hydrophobicity graphs along sequence
Bioinformatics explained: Protein hydrophobicity
Hydrophobicity scales
Pfam domain search
Download of Pfam database
Running Pfam Domain Search
Secondary structure prediction
Protein report
Protein report output
Reverse translation from protein into DNA
Reverse translation parameters
Bioinformatics explained: Reverse translation
The Genetic Code
Solving the ambiguities of reverse translation
Proteolytic cleavage detection
Proteolytic cleavage parameters
Bioinformatics explained: Proteolytic cleavage
Primers
Primer design - an introduction
General concept
Scoring primers
Setting parameters for primers and probes
Primer Parameters
Graphical display of primer information
Compact information mode
Detailed information mode
Output from primer design
Saving primers
Saving PCR fragments
Adding primer binding annotation
Standard PCR
User input
Standard PCR output table
Nested PCR
Nested PCR output table
TaqMan
TaqMan output table
Sequencing primers
Sequencing primers output table
Alignment-based primer and probe design
Specific options for alignment-based primer and probe design
Alignment based design of PCR primers
Alignment-based TaqMan probe design
Analyze primer properties
Find binding sites and create fragments
Binding parameters
Results - binding sites and fragments
Order primers
Sequencing data analyses
Importing and viewing trace data
Scaling traces
Trace settings in the Side Panel
Trim sequences
Trimming using the Trim tool
Manual trimming
Assemble sequences
Sort sequences by name
Assemble sequences to reference
Add sequences to an existing contig
View and edit read mappings
View settings in the Side Panel
Editing the read mapping
Sorting reads
Read conflicts
Using the mapping
Extract parts of a mapping
Variance table
Reassemble contig
Secondary peak calling
Cloning and cutting
Molecular cloning
Introduction to the cloning editor
The cloning workflow
Manual cloning
Insert restriction site
Gateway cloning
Add attB sites
Create entry clones (BP)
Create expression clones (LR)
Restriction site analysis
Dynamic restriction sites
Restriction site analysis from the Toolbox
Gel electrophoresis
Separate fragments of sequences on gel
Separate sequences on gel
Gel view
Restriction enzyme lists
Create enzyme list
View and modify enzyme list
Sequence alignment
Create an alignment
Gap costs
Fast or accurate alignment algorithm
Aligning alignments
Fixpoints
View alignments
Bioinformatics explained: Sequence logo
Calculation of sequence logos
Edit alignments
Move residues and gaps
Insert gaps
Delete residues and gaps
Copy annotations to other sequences
Move sequences up and down
Delete, rename and add sequences
Realign selection
Join alignments
How alignments are joined
Pairwise comparison
Pairwise comparison on alignment selection
Pairwise comparison parameters
The pairwise comparison table
Bioinformatics explained: Multiple alignments
Use of multiple alignments
Constructing multiple alignments
Phylogenetic trees
Phylogenetic tree features
Create Trees
K-mer Based Tree Construction
Create tree
Model Testing
Maximum Likelihood Phylogeny
Bioinformatics explained
Tree Settings
Minimap
Tree layout
Node settings
Label settings
Background settings
Branch layout
Bootstrap settings
Metadata
Node right click menu
Metadata and phylogenetic trees
Table Settings and Filtering
Add or modify metadata
Unknown metadata values
Selection of specific nodes
RNA structure
RNA secondary structure prediction
Selecting sequences for prediction
Structure output
Partition function
Advanced options
Structure as annotation
View and edit secondary structures
Graphical view and editing of secondary structure
Tabular view of structures and energy contributions
Symbolic representation in sequence view
Probability-based coloring
Evaluate structure hypothesis
Selecting sequences for evaluation
Probabilities
Structure scanning plot
Selecting sequences for scanning
The structure scanning result
Bioinformatics explained: RNA structure prediction by minimum free energy minimization
The algorithm
Structure elements and their energy contribution
Trimming, multiplexing and sequencing quality control
Trim Sequences
Quality trimming
Adapter trimming
Length trimming
Trim output
Demultiplex reads
Sequencing data quality control
Report contents
Adapters
Running the quality control tool
Merge overlapping pairs
Using quality scores when merging
Report of merged pairs
Tracks
Track lists
Zooming and navigating track views
Adding, removing and reordering tracks
Showing a track in a table
Open track from a track list in table view
Finding annotations on the genome
Extract sequences from tracks
Creating track lists in workflows
Retrieving reference data tracks
Merging tracks
Converting data to tracks and back
Convert to tracks
Convert from tracks
Annotate and filter tracks
Annotate with overlap information
Extract reads based on overlap
Filter annotations on name
Filter Based on Overlap
Creating graph tracks
Read mapping
Map Reads to Reference
Selecting reads and reference
Including or excluding regions (masking)
Mapping parameters
Gap placement
Computational requirements
Reference Caching
Mapping output options
Mapping reports
Detailed mapping report
Summary mapping report
Color space
Sequencing
Error modes
Mapping in color space
Viewing color space information
Mapping result
Mapping table
View settings in the Side Panel
Overlapping paired reads
Find broken pair mates
Local realignment
Method
Realignment of unaligned ends
Guided Realignment
Multi-pass local realignment
Known Limitations
Computational Requirements
How to run the Local Realignment tool
Merge mapping results
Extract consensus sequence
Coverage analysis
Running the Coverage analysis tool
Sample reads
What is Sample Reads?
How to run Sample Reads
Resequencing
Create Statistics for Target Regions
Running the Create Statistics for Target Regions
Coverage summary report
Per-region statistics
Coverage table
Coverage graph
Variant Detectors - overview
Differences among the variants called by the three variant callers
How the variant detectors work
Basic Variant Detection
Fixed Ploidy Variant Detection
Ploidy and sensitivity
Low Frequency Variant Detection
Variant Detectors - error model estimation
Variant Detectors - filters
General filters
Noise filters
Variant Detectors - the outputs
The variant track output
The annotated table output
The report
The Fixed Ploidy and Low Frequency variant callers: detailed descriptions
The Fixed Ploidy Variant caller: Models and methods
The Low Frequency Variant caller: Models and methods
InDels and Structural Variants
How to run the InDels and Structural Variants tool
The Structural Variants and InDels output
The InDels and Structural Variants detection algorithm
The InDels and Structural Variants detection algorithm - Step 1: Creating Left- and Right breakpoint signatures
The InDels and Structural Variants detection algorithm - Step 2: Creating Structural variant signatures
Theoretically expected structural variant signatures
How sequence complexity is calculated
Variant data
Variant tracks
The annotated variant table
Variant types
Special notes upgrading to Genomics Workbench 7.5
Detailed information about overlapping paired reads
Annotate and filter variants
Filter against known variants
Annotating from known variants
Annotate with exon numbers
Annotate with flanking sequence
Filter marginal variant calls
Filter reference variants
Comparing variants
Compare variants within group
Compare sample variants
Fisher exact test
Trio analysis
Filter Against Control Reads
Predicting functional consequences
Amino acid changes
Predict splice site effect
GO enrichment analysis
Conservation score annotation
Link Variants to 3D Protein Structure
Download 3D Protein Structure Database
Transcriptomics
RNA-Seq analysis
Specifying reads and reference
Tightly packed genes and genes in operons
Defining mapping options for RNA-Seq
Calculating expression values from RNA-Seq
Specifying RNA-Seq outputs
Interpreting the RNA-Seq analysis result
Small RNA analysis
Extract and count
Downloading miRBase
Annotating and merging small RNA samples
Working with the small RNA sample
Exploring novel miRNAs
Expression profiling by tags
Extract and count tags
Create virtual tag list
Annotate tag experiment
Experimental design
Setting up an experiment
Organization of the experiment table
Visualizing RNA-Seq read tracks for the experiment
Adding annotations to an experiment
Scatter plot view of an experiment
Cross-view selections
Working with tracks and experiments
Data structures for transcriptomics
From Experiments to Tracks
Running the Create Track from Experiment tool
Interpreting the results of the Create Track from Experiment tool
Transformation and normalization
Selecting transformed and normalized values for analysis
Transformation
Normalization
Quality control
Creating box plots - analyzing distributions
Hierarchical clustering of samples
Principal component analysis
Statistical analysis - identifying differential expression
Empirical analysis of DGE
Tests on proportions
Gaussian-based tests
Corrected p-values
Volcano plots - inspecting the result of the statistical analysis
Feature clustering
Hierarchical clustering of features
K-means/medoids clustering
Annotation tests
Hypergeometric tests on annotations
Gene set enrichment analysis
General plots
Histogram
MA plot
Scatter plot
De novo sequencing
De novo assembly
How it works
Resolve repeats using reads
Automatic paired distance estimation
Optimization of the graph using paired reads
AGP export
Bubble resolution
Converting the graph to contig sequences
Summary
Randomness in the results
SOLiD data support in de novo assembly
De novo assembly parameters
De novo assembly report
Map reads to contigs
Epigenomics
ChIP-Seq Analysis
Quality Control of ChIP-seq data
Learning peak shapes
Applying peak shape filters to call peaks
Running the ChIP-Seq Analysis tool
Annotate with nearby gene information
Legacy tools
Quality-based variant detection
Assessing the quality of the neighborhood bases
Significance of variant
Ploidy and genetic code
Reporting the variants
Probabilistic variant detection
Calculation of the prior and error probabilities
Calculation of the likelihood
Calculation of the posterior probability for each site type at each position in the genome
Comparison with the reference sequence and identification of candidate variants
Posterior filtering and reporting of variants
Running the variant detection
Setting ploidy and genetic code
Reporting the variants found
ChIP-Seq Analysis (legacy)
Peak finding and false discovery rates
Read shifting
Peak refinement
Reporting the results
Appendix
Comparison of workbenches
Use of multi-core computers
Graph preferences
BLAST databases
Peptide sequence databases
Nucleotide sequence databases
Adding more databases
Proteolytic cleavage enzymes
Restriction enzymes database configuration
Technical information about modifying Gateway cloning sites
IUPAC codes for amino acids
IUPAC codes for nucleotides
Formats for import and export
List of bioinformatic data formats
List of graphics data formats
SAM/BAM export format specification
Flags
Gene expression annotation files and microarray data formats
GEO (Gene Expression Omnibus)
Affymetrix GeneChip
Illumina BeadChip
Gene ontology annotation files
Generic expression and annotation data file formats
Translation Tables
1. Standard
2. Vertebrate Mitochondrial
3. Yeast Mitochondrial
4. Mold Mitochondrial; Protozoan Mitochondrial; Coelenterate Mitochondrial; Mycoplasma; Spiroplasma
5. Invertebrate Mitochondrial
6. Ciliate Nuclear; Dasycladacean Nuclear; Hexamita Nuclear
9. Echinoderm Mitochondrial; Flatworm Mitochondrial
10. Euplotid Nuclear
11. Bacterial and Plant Plastid
12. Alternative Yeast Nuclear
13. Ascidian Mitochondrial
14. Alternative Flatworm Mitochondrial
15. Blepharisma Macronuclear
16. Chlorophycean Mitochondrial
21. Trematode Mitochondrial
22. Scenedesmus Obliquus Mitochondrial
23. Thraustochytrium Mitochondrial
24. Pterobranchia Mitochondrial
25. Candidate Division SR1 and Gracilibacteria
Custom codon frequency tables
Comparison of track comparison tools
Matrices for alignment calculation
PAM30 log-odds matrix
PAM60 log-odds matrix
BLOSUM42 log-odds matrix
BLOSUM62 log-odds matrix
BLOSUM80 log-odds matrix
Bibliography
Quick search history
You can access the 10 most recent searches by clicking the icon (
) next to the search field (see figure
3.22
).
Figure
3
.
22
:
Recent searches.
Clicking one of the recent searches will conduct the search again.