Analyze QIAseq miRNA
The Analyze QIAseq miRNA (Illumina) and the Analyze QIAseq miRNA (Ion Torrent) template workflows can be used to analyze data produced with the QIAseq miRNA Library Kit.
The workflows include all necessary steps for processing and analyzing the reads:
- Reads are trimmed using Trim Reads.
Note that trimming is only performed for Ion Torrent reads, as Illumina reads do not have the 5' adapter.
- UMI reads are created using Create UMI Reads for miRNA.
- Expression is quantified using Quantify miRNA.
Launching the workflows
To run these workflows, go to
Workflows | Template Workflows | Biomedical Workflows (
) | QIAseq Sample Analysis (
) | QIAseq RNA Workflows (
)
and select:
Analyze QIAseq miRNA (Illumina) (
)
Analyze QIAseq miRNA (Ion Tirrent) ()
See Launching workflows individually and in batches for general information.
Options can be configured in the following wizard steps:
- Choose where to run. If you are connected to a CLC Server via the CLC Workbench, you will be asked where you would like to run the analysis. We recommend that you run the analysis on a CLC Server when possible.
- Select Reads. Select the input reads. When analyzing more than one sample at a time, check Batch in the lower left corner of the dialog.
- Specify reference data handling. Select the QIAseq Small RNA Reference Data Set, see Reference data management for details.
- Configure batching, if running the workflow in batch mode. Define the batch units.
- Batch overview, if running the workflow in batch mode. Verify that the batching is as intended.
- Quantify miRNA. Choose whether to enable spike-in analysis and which miRBase database to use. The list of species used for annotations can be prioritized, with the sequenced species placed first. See Quantify miRNA for details.
- Result handling. Choose if a workflow result metadata and/or log should be saved.
- Save location for new elements. Choose where to save the data, and press Finish to start the analysis.
Launching using the QIAseq Panel Analysis Assistant
The workflows are also available in the QIAseq Panel Analysis Assistant under miRNA.
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