Primer design can be accessed in two ways:
select sequence | Toolbox in the Menu Bar | Molecular Biology Tools () | Primers and Probes ()| Design Primers () | OK
or right-click sequence | Show | Primer ()
Note! This means that annotations such as e.g. known SNP's or exons can be displayed on the template sequence to guide the choice of primer regions. Also, traces in sequencing reads can be shown along with the structure to guide e.g. the re-sequencing of poorly resolved regions.