Basic analysis

The basic analysis section assesses the most simple characteristics that are supported by all sequencing technologies.
Sequence length distribution
Calculates absolute amounts of sequences that have been observed for individual sequence lengths in base-pairs. The resulting table correlates sequence-lengths in base-pairs with numbers of sequences observed with that number of base-pairs.
Base coverage distribution
Calculates absolute coverages for individual base positions, which is obviously very similar to the sequence length distribution. The resulting table correlates base-positions with the number of sequences that supported (covered) that position.
Sequence-wise %GC-content distribution
Calculates absolute amounts of sequences that feature individual %GC-contents in 101 bins ranging from 0 to 100%.The %GC-content of a sequence is calculated by dividing the absolute number of G/C-nulceotides by the length of that sequence.
Sequence-wise %N-content distribution
Calculates the absolute amount of sequences that feature individual %N-contents in 101 bins ranging from 0 to 100%, where N refers to all ambiguous base-codes as specified by IUPAC.The %N-content of a sequence is calculated by dividing the absolute number of ambiguous nucleotides through the length of that sequence.
Base-wise nucleotide distributions
Calculates absolute coverages for the four DNA nucleotides (A, C, G or T) throughout the individual base-positions.
Base-wise GC-distribution
Calculates absolute coverages of C's + G's throughout individual base-positions
Base-wise N-distribution
Calculates absolute coverages of N's, throughout individual base-positions, where N refers to all ambiguous base-codes as specified by IUPAC.