Illumina BeadChip
Both BeadChip expression data files from Illumina's BeadStudio software and the corresponding BeadChip annotation files are supported by CLC Genomics Workbench. The formats of the BeadStudio and annotation files have changed somewhat over time and various formats are supported.
Illumina expression data, compact format
An example of this format is shown below:
TargetID AVG_Signal BEAD_STDEV Detection GI_10047089-S 112.5 4.2 0.16903226 GI_10047091-S 127.6 4.8 0.76774194All this information is imported into the Workbench. The
AVG_Signal
is used as the expression measure.
Download a small sample file here:
https://resources.qiagenbioinformatics.com/madata/IlluminaBeadChipCompact.txt
Illumina expression data, extended format
An example of this format is shown below:
TargetID MIN_Signal AVG_Signal MAX_Signal NARRAYS ARRAY_STDEV BEAD_STDEV Avg_NBEADS Detection GI_10047089-S 73.7 73.7 73.7 1 NaN 3.4 53 0.05669084 GI_10047091-S 312.7 312.7 312.7 1 NaN 11.1 50 0.99604483All this information is imported into the Workbench. The
AVG_Signal
is used as the expression measure.
Download a small sample file here:
https://resources.qiagenbioinformatics.com/madata/IlluminaBeadChipExtended.txt
Illumina expression data, with annotations
An example of this format is shown below:
TargetID Accession Symbol Definition Synonym Signal-BG02 DCp32 Detection-BG02 DCp32 GI_10047089-S NM_014332.1 SMPX "Homo sapiens small muscle protein, X-linked (SMPX), mRNA." -17.6 0.03559657 GI_10047091-S NM_013259.1 NP25 "Homo sapiens neuronal protein (NP25), mRNA." NP22 32.6 0.99604483 GI_10047093-S NM_016299.1 HSP70-4 "Homo sapiens likely ortholog of mouse heat shock protein, 70 kDa 4 (HSP70-4), mRNA." 228.1 1Only the TargetID, Signal and Detection columns will be imported, the remaining columns will be ignored. This means that the annotations are not imported. The
Signal
is used as the expression measure.
Download a small example sample file here:
https://resources.qiagenbioinformatics.com/madata/IlluminaBeadStudioWithAnnotations.txt
Illumina expression data, multiple samples in one file
This file format has too much information to show it inline in the text. You can download a small example sample file here:
https://resources.qiagenbioinformatics.com/madata/IlluminaBeadStudioMultipleSamples.txt
This file contains data for 18 samples. Each sample has an expression value (the value in the AVG_Signal
column), a detection p-value, a bead standard deviation and an average bead number column. The workbench recognizes the 18 samples and their columns.
Illumina annotation files
The Workbench supports import of two types of Illumina BeadChip annotation files. These are either comma-separated or tab-delimited .txt files. They can be used to annotate experiments as shown in Adding annotations.
This file format has too much information to show it inline in the text.
Download a small example annotation file of the first type here:
https://resources.qiagenbioinformatics.com/madata/IlluminaBeadChipAnnotation.txt