Create Heat Map for RNA-Seq

The Create Heat Map tool simultaneously clusters samples and features, showing a two dimensional heat map of expression values. Each column corresponds to one sample, and each row corresponds to a feature (a gene or a transcript). The samples and features are both hierarchically clustered. Known metadata about each sample is added as an overlay. In addition, the following filtering and normalization are performed:

For more detail about these steps, see RNA-Seq normalization.



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