In CLC Genomics Workbench you can create a graph in the electric charge of a protein as a function of pH. This is particularly useful for finding the net charge of the protein at a given pH. This knowledge can be used e.g. in relation to isoelectric focusing on the first dimension of 2D-gel electrophoresis. The isoelectric point (pI) is found where the net charge of the protein is zero. The calculation of the protein charge does not include knowledge about any potential post-translational modifications the protein may have.
The pKa values reported in the literature may differ slightly, thus resulting in different looking graphs of the protein charge plot compared to other programs.
In order to calculate the protein charge:
Toolbox | Classical Sequence Analysis () | Protein Analysis ()| Create Protein Charge Plot ()
This opens the dialog displayed in figure 15.1:
If a sequence was selected before choosing the Toolbox action, the sequence is now listed in the Selected Elements window of the dialog. Use the arrows to add or remove sequences or sequence lists from the selected elements.
You can perform the analysis on several protein sequences at a time. This will result in one output graph showing protein charge graphs for the individual proteins.
Click Finish to start the tool.
Figure 15.2 shows the electrical charges for three proteins. In the Side Panel to the right, you can modify the layout of the graph.
See Graph preferences for information about the graph view.