Compare Cell Clonotypes
Compare Cell Clonotypes contrasts properties, such as diversity and similarity, of the immune repertoires identified for groups of cells, as determined by the sample or, when available, through Cell Clusters (
) or Cell Annotations (
).
The Compare Cell Clonotypes tool can be found in the Toolbox here:
Toolbox | Single Cell Analysis (
) | Immune Repertoire (
) | Compare Cell Clonotypes (
)
The tool takes a TCR Cell Clonotypes (
) or BCR Cell Clonotypes (
) element as input. If the clonotypes to be compared are found in different elements, these can be combined using the Combine Cell Clonotypes tool, see Combine Cell Clonotypes for details.
The following options can be adjusted (figure 13.5):
Figure 13.5: The default options in the dialog of the Compare Cell Clonotypes tool. Note that "Group by" needs to contain at least one value before proceeding. This can always be set to "Sample".
- Clusters and Cell annotations (Optional). Clusters accepts Cell Clusters () and Cell annotations accepts Cell Annotations (). These can be created within the CLC Single Cell Analysis Module for scRNA-Seq data, but they can also be imported, see Import Cell Annotations and Import Cell Clusters for details.
- Group by. Any categories from the supplied Cell Clusters or Cell Annotations. "Sample" can be additionally chosen, even if no Cell Clusters or Cell Annotations are provided.
If Cell Annotations contained a category `Infection' with values `Pre' and `Post', and another category `Individual' with values `Ind1' and `Ind2', then selecting Group by = Infection, Individual would give groups `Pre - Ind1', `Pre - Ind2', `Post - Ind1' and `Post - Ind2'.
The chosen options need to induce at least two groups of cells. Otherwise, the tool will fail with a relevant message.
Note that category from Cell Annotations that only contain non-integer numerical data are not supported.
- Select groups (Optional). This can be supplied to reduce the number of groups of cells in the outputs to only those of interest, or to control the order in which the groups are shown. For example, if the aim is to investigate the infection effect in individual 1, the `Pre - Ind1' and `Post - Ind1' groups can be selected. If left empty, all groups will be used.
Subsections