View and edit contigs and read mappings

The results of the assembly or mapping (assembly to a reference) are respectively contigs or a read mapping. In both cases the sequence reads have been aligned (see figure 18.12). If multiple reference sequences were used, this information will be in a table where the actual visual mapping can be opened by double-clicking.

Image initialcontigview
Figure 18.12: The view of a contig. Note that you can zoom to a very detailed level.

You can see that color of the residues and trace at the end of one of the reads has been faded. This indicates that this region has not contributed to the contig or mapping. This may be due to trimming before or during the assembly or to misalignment to the other reads.

You can easily adjust the trimmed area to include more of the read in the contig or mapping: simply drag the edge of the faded area as shown in figure 18.13.

Image dragtrimmedregion
Figure 18.13: Dragging the edge of the faded area.

Note! The handles for dragging are only available at the zoom level where residues can be seen. This means that you need to have zoomed in to 100% or more and chosen Compactness levels "Not compact", "Low" or "Packed".

Residues are colored green unless they were reversed to map. in which case they will be red. The colors can be changed in the Side Panel as described in View settings in the Side Panel

If you find out that the reversed reads should have been the forward reads and vice versa, you can reverse complement the whole contig or mapping. Right-click in the empty white area of the contig or mapping and choose to Reverse Complement Sequence.


Subsections