Introduction to the cloning editor

In the cloning editor, most of the basic options for viewing, selecting and zooming the sequences are the same as for the standard sequence view. See View sequences for an explanation of these options. This means that e.g. known SNP's, exons and other annotations can be displayed on the sequences to guide the choice of regions to clone.

However, the cloning editor has a special layout with three distinct areas (in addition to the Side Panel found in other sequence views as well):

There are essentially three ways of performing cloning in the Biomedical Genomics Workbench. The first is the most straight-forward approach, which is based on a simple model of selecting restriction sites for cutting out one or more fragments and defining how to open the vector to insert the fragments. This is described as The cloning workflow. The second approach is unguided and more flexible and allows you to manually cut, copy, insert and replace parts of the sequences. This approach is described under Manual cloning. Finally, the Biomedical Genomics Workbench also supports Gateway cloning.