UMI
- Mapped read pairs The number of mapped read pairs including specific, non-specific and broken read pairs before UMI grouping.
- UMI read pairs Read pairs after UMI grouping.
- Singleton UMI read pairs UMI read pairs generated from only one read pair. The percentage is calculated relative to "UMI read pairs".
- Simplex UMI read pairs UMI read pairs where input reads all originate from the same strand. Singleton UMI read pairs are a subset of the simplex UMI read pairs. The percentage is calculated relative to "UMI read pairs".
- Duplex UMI read pairs UMI read pairs that are based on input reads from both strands. The percentage is calculated relative to "UMI read pairs".
- Proper UMI read pairs UMI read pairs that are mapped as pairs. The percentage is calculated relative to "UMI read pairs".
- Broken UMI read pairs UMI read pairs where the distance between the individual reads in the pair exceeded the expected distance for paired reads, or where only one of the reads in the pair was mapped. The percentage is calculated relative to "UMI read pairs".
- Specific proper UMI read pairs UMI read pairs that are mapped as pairs and are specific. The percentage is calculated relative to "UMI read pairs".
- Non-specific proper UMI read pairs UMI read pairs that are mapped as pairs, but are non-specific. The percentage is calculated relative to "UMI read pairs".
- Average number of reads per UMI The average number of read pairs per UMI read pair.
- Median number of reads per UMI The median number of read pairs per UMI read pair.
- Average number of reads per duplex UMI The average number of read pairs per duplex UMI read pair.
- Median number of reads per duplex UMI The median number of read pairs per duplex UMI read pair.
The plot Reads by group size shows the number of proper input read pairs distributed by the size of the UMI groups that they have been grouped to. When running duplex UMI analyses, input read pairs that are grouped to duplex UMI read pairs are not represented in this plot.
The plot Groups by group size shows the number of UMI groups distributed by the UMI group sizes. When running duplex UMI analyses, duplex groups are not represented in this plot.
The plot Reads by group size (duplex) shows the number of proper input read pairs distributed by the size of the duplex UMI groups that they have been grouped to. If reads are not assigned to a duplex UMI group, they are not represented in this plot.
The plot Groups by group size (duplex) shows the number of duplex UMI groups distributed by the duplex UMI group sizes.