Definitions
- Duplex UMI A protocol where both read 1 and read 2 in a pair contain a UMI. Reads originating from both strands of a DNA fragment can be grouped.
- Singleton UMI read pairs A UMI read pair that is based on only one input read pair.
- Simplex UMI read pairs For duplex protocols, the number of simplex UMI read pairs is provided. Simplex UMI read pairs are UMI read pairs where input reads all originate from the same strand. Singleton UMI read pairs are a subset of the simplex UMI read pairs.
- Duplex UMI read pairs UMI read pairs that are based on input reads from both strands.