Input read QC
This section contains information about the input reads before quality trimming.
For the per-sequence analysis, average quality of a read is calculated from the arithmetic mean of it's error probabilities.
- Per-sequence analysis
- Read N50 The read length that 50% of reads are longer than or equal to.
- Average read length Average length of input reads.
- Median read length Median length of input reads.
- Average quality Average quality of input reads.
- Median quality Median quality of input reads.
- Lengths distribution Plot showing the distribution of read lengths.
- Quality distribution Plot showing the distribution of average quality scores.
- Reads passing average quality thresholds Table providing the percentage of reads with average quality above 20, 25, 30 and 35.
- Per-base analysis
- Bases passing average quality thresholds Table providing the percentage of bases with average quality above 20, 25, 30 and 35.