Restriction enzyme lists
CLC Main Workbench includes restriction enzymes available in the REBASE database, with methylation shown as performed by the cognate methylase rather than by Dam/Dcm. If you want to customize the enzyme database for your installation, see Restriction enzymes database configuration. However, when performing restriction site analyses, it is often an advantage to use a customized list of enzymes. In this case, the user can create special lists containing for example all enzymes available in the laboratory freezer, or all enzymes used to create a given restriction map or all enzymes that are available form the preferred vendor.
In the Example data (import in your Navigation Area using the Help menu), under Nucleotide->Restriction analysis, there are two enzyme lists: one with the 50 most popular enzymes, and another with all enzymes that are included in the CLC Main Workbench.
Create enzyme list
CLC Main Workbench uses enzymes from the REBASE restriction enzyme database at http://rebase.neb.com. If you want to customize the enzyme database for your installation, see Restriction enzymes database configuration.
To create an enzyme list of a subset of these enzymes:
File | New |
Enzyme list (
)
This opens the dialog shown in figure 23.16
Figure 23.16: Choosing enzymes for the new enzyme list.
Choose which enzyme you want to include in the new enzyme list (see Manage enzymes), and click Finish to open the enzyme list.
View and modify enzyme list
An enzyme list is shown in figure 23.17. It can be sorted by clicking the columns, and you can use the filter at the top right corner to search for specific enzymes, recognition sequences etc.
If you wish to remove or add enzymes, click the Add/Remove Enzymes button at the bottom of the view. This will present the same dialog as shown in figure 23.16 with the enzyme list shown to the right.
If you wish to extract a subset of an enzyme list, open the list, select the relevant enzymes, right-click on the selection and choose to Create New Enzyme List from
Selection ().
If you combined this method with the filter located at the top of the view, you can extract a very specific set of enzymes. for example, if you wish to create a list of enzymes sold by a particular distributor, type the name of the distributor into the filter and select and create a new enzyme list from the selection.