Proteolytic cleavage enzymes

Most proteolytic enzymes cleave at distinct patterns. Below is a compiled list of proteolytic enzymes used in CLC Main Workbench.

Name P4 P3 P2 P1 P1' P2'
Cyanogen bromide (CNBr) - - - M - -
Asp-N endopeptidase - - - - D -
Arg-C - - - R - -
Lys-C - - - K - -
Trypsin - - - K, R not P -
Trypsin - - W K P -
Trypsin - - M R P -
Trypsin* - - C, D K D -
Trypsin* - - C K H, Y -
Trypsin* - - C R K -
Trypsin* - - R R H,R -
Chymotrypsin-high spec. - - - F, Y not P -
Chymotrypsin-high spec. - - - W not M, P -
Chymotrypsin-low spec. - - - F, L, Y not P -
Chymotrypsin-low spec. - - - W not M, P -
Chymotrypsin-low spec. - - - M not P, Y -
Chymotrypsin-low spec. - - - H not D, M, P, W -
o-Iodosobenzoate - - - W - -
Thermolysin - - - not D, E A, F, I, L, M or V -
Post-Pro - - H, K, R P not P -
Glu-C - - - E - -
Asp-N - - - - D -
Proteinase K - - - A, E, F, I, L, T, V, W, Y - -
Factor Xa A, F, G, I, L, T, V, M D,E G R - -
Granzyme B I E P D - -
Thrombin - - G R G -
Thrombin A, F, G, I, L, T, V, M A, F, G, I, L, T, V, W, A P R not D, E not D, E
TEV (Tobacco Etch Virus) - Y - Q G, S -