Import Roche 454

This tool has been deprecated and will be retired in a future version of the software. It has been moved to the Legacy Tools (Image legacy_tools) folder of the Toolbox, and its name has "(legacy)" appended to it. If you have concerns about the future retirement of this tool, please contact QIAGEN Bioinformatics Support team at [email protected].

To open the Roche 454 Import tool, go to :

        Legacy Tools (Image legacy_tool_closed_16_n_p) | Roche 454

Choosing the Roche 454 import will open the dialog shown in figure 35.4.

Image importngsdialog-454
Figure 35.4: Importing data from Roche 454.

We support import of two kinds of data from 454 GS FLX systems:

The General options to the left are:

Image 454-linker-preferences
Figure 35.5: Specifying linkers for 454 import.

Note! During import, partial adapter sequences are removed (TCAG and ATGC ), and if the full sequencing adapters GCCTTGCCAGCCCGCTCAG , GCCTCCCTCGCGCCATCAG or their reverse complements are found, they are also removed (including tailing Ns). If you do not wish to remove the adapter sequences (e.g. if they have already been removed by other software), please uncheck the Remove adapter sequence option.

Click Next to adjust how to handle the results). We recommend choosing Save in order to save the results directly to a folder, since you probably want to save anyway before proceeding with your analysis. There is an option to put the import data into a separate folder. This can be handy for better organizing subsequent analysis results and for batch processing.