Find binding sites and create fragments

In CLC Genomics Workbench you have the possibility of matching known primers against one or more DNA sequences or a list of DNA sequences. This can be applied to test whether a primer used in a previous experiment is applicable to amplify a homologous region in another species, or to test for potential mispriming. This functionality can also be used to extract the resulting PCR product when two primers are matched. This is particularly useful if your primers have extensions in the 5' end. Note that this tool is not meant to analyze rapidly high-throughput data. The maximum amount of sequences the tool will handle in a reasonable amount of time depends on your computer processing capabilities.

To search for primer binding sites:

        Toolbox | Molecular Biology Tools (Image lab_work_support) | Primers and Probes (Image primersandprobes)| Find Binding Sites and Create Fragments (Image searchforprimers)

If a sequence was already selected in the Navigation Area, this sequence is now listed in the Selected Elements window of the dialog. Use the arrows to add or remove sequences or sequence lists from the selected elements.

Click Next when all the sequence have been added.

Note! You should not add the primer sequences at this step.