Gateway cloning

CLC Main Workbench offers tools to perform in silico Gateway cloning (Thermo Fisher Scientific), including Multi-site Gateway cloning.

The three tools for doing Gateway cloning in the CLC Main Workbench mimic the procedure followed in the lab:

During this process, both the attB-flanked fragment and the entry clone can be saved.

For more information about the Gateway technology, please visit http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning/gateway-technology.html. To perform these analyses in CLC Main Workbench, you need to import donor and expression vectors. These can be found on the Thermo Fisher Scientific's website: find the relevant vector sequences, copy them, and paste them in the field that opens when you choose New | Sequence in the workbench. Fill in additional information appropriately (enter a "Name", check the "Circular" option) and save the sequences in the Navigation Area.



Subsections