Gateway cloning

CLC Main Workbench offers tools to perform in silico Gateway cloning (Thermo Fisher Scientific), including Multi-site Gateway cloning.

The three tools for doing Gateway cloning in the CLC Main Workbench mimic the procedure followed in the lab:

During this process, both the attB-flanked fragment and the entry clone can be saved.

For more information about the Gateway technology, please visit To perform these analyses in CLC Main Workbench, you need to import donor and expression vectors. These can be found on the Thermo Fisher Scientific's website: find the relevant vector sequences, copy them, and paste them in the field that opens when you choose New | Sequence in the workbench. Fill in additional information appropriately (enter a "Name", check the "Circular" option) and save the sequences in the Navigation Area.