Output from the Quantify QIAseq UPX 3' workflow
The Quantify QIAseq UPX 3' workflow generates the following outputs:
- A Gene Expression track (
) A track with gene expression annotations, including counts and expression values for each gene. Hovering the mouse over an individual annotation reveals a tooltip with the gene name, expression value and associated information. Further details about this track type can be found at http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Expression_tracks.html.
Gene expression tracks can be used in subsequent analyses using tools under the RNA-Seq Analysis and Small RNA Analysis folder of the Toolbox. They can also be used in workflows such as the Detect Differentially Expressed Genes Between Two Groups template workflows, which can be launched using the Analyze QIAseq Samples guide.
- Two trimming reports See http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Trim_output.html. One is generated after trimming short polyA sequences from the middle of R1 and discarding R2. The second is issued after trimming polyA from R1.
- A UMI Group report (
) A report containing a breakdown of UMI groups with different number of reads, along with percentage of groups and reads (see Calculate Unique Molecular Index Groups).
- A UMI Group Creation report () A report with information about how many reads were ignored and the reason why they were not included in a UMI read (see Create UMI Reads from Grouped Reads).
- An RNA-Seq report () See http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=RNA_Seq_report.html.
Note that the Combine Reports tool can be used to combine multiple reports, summarizing results to allow for quality checks across samples. See http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Combine_Reports.html for further details.