Perform QIAseq FastSelect Rna Expression and Fusion Calling (N6-T RT primer)

The Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT primer) template workflow supports a N6-T RT primer protocol. The Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT primer) workflow can be found here:

        Template Workflows | Biomedical Workflows (Image biomedical_twf_folder_open_16_n_p) | QIAseq Sample Analysis (Image qiaseqrna_folder_closed_16_n_p) | QIAseq RNA Workflows (Image qiaseq_workflows_folder_closed_16_n_p) | Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT primer) (Image quantify_qiaseq_upx_16_n_p)

Or run directly from the Analyze QIAseq Samples guide:

        Template Workflows | Biomedical Workflows (Image biomedical_twf_folder_open_16_n_p) | QIAseq Sample Analysis (Image qiaseqrna_folder_closed_16_n_p) | Analyze QIAseq Samples (Image qiaseq_panel_guide_16_h_p)

where it is available in the dropdown menu of the FastSelect RNA tab by setting analysis to N6-T RT primer.

In the first wizard step either select the reads or choose Select files for import to import FASTQ files on-the-fly. Note that the FASTQ files need to be imported upfront for Analyze QIAseq Samples to work.

The workflow is designed to analyze all samples within an experiment without the need of batch functionality. It iterates through the samples and processes them separately, and collects and distributes all outputs at the end.

Choose the QIAseq UPXome and FastSelect RNA hg38 reference data when running the workflow standalone.

Keep the "Use organization of input samples" except when you want to add metadata information to the samples. Check that the batch overview looks as expected, especially when using on-the-fly import.

Save the results to a specified location.



Subsections