RNA-seq Analysis Portal analysis explained

RNA-seq Analysis Portalfacilitates analysis of FASTQ or BAM data generated on Illumina® or Themo Fisher® instruments for the appropriate sample kits listed under Supported sample kits.

This chapter describes the secondary analysis, which for all sample kit types consists of two steps handled by separate analysis workflows:

  1. Align and count - alignment and counting of sequencing reads. Outputs are samples containing expression counts.
  2. Create experiment - calculation of differential expression between groups of samples. Outputs are one or more differential expression analyses.

The details of the two analysis workflows differ for different sample kits. Details of these differences are described in the sections below.

In addition, this chapter covers Taxonomic profiling of unmapped reads and Comparison of differential expression analyses.


Subsections