• Product manuals

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• Introduction
• General Description
  • Supported sample kits and sequencing platforms
  • Accessing RNA-seq Analysis Portal
  • Analysis credits for RNA-seq Analysis Portal
• Getting Started
  • Upload sequencing data
  • Align and count
  • Create experiment
  • View and filter results
  • What's next - send results downstream
  • Design follow-up experiments in GeneGlobe
• User Interface
  • Analysis Portal page
    • Front page and Projects overview
    • Project view
    • Send copy of project
  • Samples page
• Starting Analyses
  • Align and count dialog
    • QIAseq UPX 3' Transcriptome Kit and QIAseq UPXome RNA Lib Kit specific settings
  • Create experiment dialog
    • Sample grouping
    • Experimental design
  • Compare analyses dialog
• Viewing Results
  • Experiment summary and QC report
  • Differential expression view
    • Feature table
    • Volcano plot
    • Heat map
    • Biological insights table
    • Filter
    • Send results to GeneGlobe
    • Send results to QIAGEN IPA
  • Comparison view
• RNA-seq Analysis Portal analysis explained
  • Align and count analysis workflows
    • Align and count - QIAseq miRNA Library Kit
    • Align and count - QIAseq UPX 3' Transcriptome Kit
    • Align and count - QIAseq UPXome RNA Lib Kit
    • Align and count - Demultiplexed RNA sample kits
  • Create experiment analysis workflow
  • Taxonomic profiling of unmapped reads
  • Compare analyses
• Appendix A - Sequence file naming pattern
• Appendix B - Reference and annotation information
  • General references and annotations
  • Sample kit specific resources
• Appendix C - Analysis workflow versions, species support and parameters
  • QIAseq miRNA Library Kit (Illumina)
  • QIAseq miRNA Library Kit (Thermo Fisher)
  • QIAseq UPX 3' Transcriptome Kit
  • QIAseq UPXome RNA Lib Kit
  • QIAseq UPXome RNA Lib Kit (N6-T RT primer)
  • QIAseq UPXome RNA Lib Kit (ODT-T RT primer)
  • QIAseq UPXome RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq FastSelect RNA Lib Kit (N6-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (ODT-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq Stranded mRNA Select/Stranded Total RNA Lib Kit
  • TruSeq Stranded Total RNA Library Prep (Human/Rat, Gold, Globin)
  • Illumina Stranded Total RNA Prep with Ribo-Zero Plus
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  • KAPA RNA HyperPrep Kit
  • SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian/Low Input Mammalian
  • Collibri Stranded RNA Library Prep Kit for Illumina Systems
• Appendix D - RNA-seq Analysis Portal for QIAGEN IPA users
• Appendix E - Quality control details
  • QIAseq miRNA Library Kits
  • QIAseq UPX 3' Transcriptome Kits
  • QIAseq Stranded RNA Library Kits (FastSelect RNA Library Kit, Stranded mRNA/Stranded Total RNA Library Kits), QIAseq UPXome Kit, and all third-party kits

Sample kit specific resources

QIAseq miRNA Library Kit

• miRBase: mirbase_v22, http://mirbase.org/
• spike-ins: QIAseq miRNA Library QC Spike-ins
• piRNA: https://www.pirnadb.org/
  • Caenorhabditis elegans (WBcel235.105): piRNAdb.cel.v1_7_6
  • Drosophila melanogaster (BDGP6.32.105): piRNAdb.cel.v1_7_6
  • Homo sapiens (GRCh38.103): piRNAdb.hsa.v1_7_6
  • Mus musculus (GRCm38.101): piRNAdb.mmu.v1_7_6
  • Rattus norvegicus (Rnor6.0.103): piRNAdb.rno.v1_7_6
  • Rattus norvegicus (mRatBN7.2.110): piRNAdb.rno.v1_7_6
• GtRNAdb: http://gtrnadb.ucsc.edu/
  Applied to analysis workflow versions v1.0 and v1.1 only.
  • Homo sapiens (GRCh38.103): hg38-tRNAs.fa, release 18
  • Mus musculus (GRCm38.101): mm10-tRNAs.fa,release 18
  • Rattus norvegicus (Rnor6.0.103): rn6-tRNAs.fa, release 18

Stranded mRNA Select Kit, QIAseq Stranded Total RNA Lib Kit, and QIAseq UPX 3' Transcriptome Kit

• Spike-ins: ERCC RNA spike-ins, https://shop.nist.gov/ccrz__ProductDetails?sku=2374&cclcl

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