• Product manuals

Browse the manual

• Introduction
• General Description
  • Supported sample kits
  • Accessing RNA-seq Analysis Portal
  • Analysis credits for RNA-seq Analysis Portal
• Getting Started
  • Upload sequencing data
  • Align and count
  • Create experiment
  • View and filter results
  • Next steps in GeneGlobe
  • Send to QIAGEN IPA
• User Interface
  • Analysis Portal page
    • Front page and Projects overview
    • Project view
    • Send copy of project
  • Samples page
• Starting Analyses
  • Align and count dialog
    • QIAseq UPX 3' Transcriptome Kit and QIAseq UPXome RNA Lib Kit specific settings
  • Create experiment dialog
    • Sample grouping
    • Experimental design
  • Compare analyses dialog
• Viewing Results
  • Experiment summary and QC report
  • Differential expression view
    • Feature table
    • Volcano plot
    • Heat map
    • Biological insights table
    • Filter
    • What's next - My QIAGEN users
    • Send to QIAGEN IPA - QIAGEN IPA users
  • Comparison view
• RNA-seq Analysis Portal analysis explained
  • Align and count analysis workflows
    • Align and count - QIAseq miRNA Library Kit
    • Align and count - QIAseq UPX 3' Transcriptome Kit
    • Align and count - QIAseq UPXome RNA Lib Kit
    • Align and count - Demultiplexed RNA sample kits
  • Create experiment analysis workflow
  • Taxonomic profiling of unmapped reads
  • Compare analyses
• Appendix A - Sequence file naming pattern
• Appendix B - Reference and annotation information
  • General references and annotations
  • Sample kit specific resources
• Appendix C - Analysis workflow versions, species support and parameters
  • QIAseq miRNA Library Kit (Illumina)
  • QIAseq miRNA Library Kit (Thermo Fisher)
  • QIAseq UPX 3' Transcriptome Kit
  • QIAseq UPXome RNA Lib Kit
  • QIAseq UPXome RNA Lib Kit (N6-T RT primer)
  • QIAseq UPXome RNA Lib Kit (ODT-T RT primer)
  • QIAseq UPXome RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq FastSelect RNA Lib Kit (N6-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (ODT-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq Stranded mRNA Select/Stranded Total RNA Lib Kit
  • TruSeq Stranded Total RNA Library Prep (Human/Rat, Gold, Globin)
  • Illumina Stranded Total RNA Prep with Ribo-Zero Plus
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  • KAPA RNA HyperPrep Kit
  • SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian/Low Input Mammalian
  • Collibri Stranded RNA Library Prep Kit for Illumina Systems
• Appendix D - RNA-seq Analysis Portal for QIAGEN IPA users
  • Sending results to QIAGEN IPA
  • Further analysis in QIAGEN IPA
  • How to get credits as an IPA usersA
• Appendix E - Quality control details
  • QIAseq miRNA Library Kits
  • QIAseq UPX 3' Transcriptome Kits
  • QIAseq Stranded RNA Library Kits (FastSelect RNA Library Kit, Stranded mRNA/Stranded Total RNA Library Kits), QIAseq UPXome Kit, and all third-party kits

Align and count - QIAseq miRNA Library Kit

The analysis workflow is based on the Biomedical Genomics Analysis QIAseq miRNA Quantification analysis workflow described at

https://resources.qiagenbioinformatics.com/manuals/biomedicalgenomicsanalysis/2101/index.php?manual=QIAseq_miRNA_Quantification.html

It includes the steps below. Specific parameters are listed in Appendix C.

• Create UMI Reads for miRNA. Sequencing reads are merged to UMI reads based on the unique molecular indexes (UMIs).
• Quantify miRNA. miRNAs are counted and annotated using miRBase and custom databases. Expression levels are generated for the mature miRNAs.

The tools are described in more detail in the QIAGEN CLC Genomics Workbench and Biomedical Genomics Analysis plugin manuals:

• https://resources.qiagenbioinformatics.com/manuals/biomedicalgenomicsanalysis/2101/index.php?manual=Create_UMI_Reads_miRNA.html
• https://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/2103/index.php?manual=Quantify_miRNA.html

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