QIAseq miRNA Library Kit (Thermo Fisher)
The QIAseq miRNA Library Kit (Thermo Fisher) analysis workflow is based on miRBase, why only species present in miRBase are supported.Ingenuity Pathway Analysis for miRNA data is restricted to human, mouse and rat. For this reason, biological insights results will be generated for these species only.
For an overview of what species are supported for the QIAseq miRNA Library Kit (Thermo Fisher), see About analysis workflow versions and sample comparability.
Analysis workflow versions
- v1.0 - RNA-seq Analysis Portal 5.0
Align and count analysis workflow parameters
Only applied options and parameter are listed.
| Trim Reads | |
| Adapter trimming by Trim adapter list | 5prime_Ion_Torrent_miRNA_trim_adapter |
| Create UMI Reads for miRNA | |
| Minimum length of miRNA | 15 |
| Maximum length of miRNA | 55 |
| UMI length | 12 |
| Maximum size of small UMI groups | 1 |
| Common sequence | AACTGTAGGCACCATCAAT |
| Maximum differences in common sequence | 1 |
| Quantify miRNA | |
| Minimum supporting count | 1 |
| Prioritized species | Selected species |
| Allow length-based isomiRs | Yes |
| - Additional upstream bases | 2 |
| - Additional downstream bases | 2 |
| - Missing upstream bases | 2 |
| - Missing downstream bases | 2 |
| Maximum mismatches | 2 |
| Strand specific | Yes |
| Minimum sequence length | 18 |
| Maximum sequence length | 25 |
| Annotation records | v1.0: miRBase, piRNA (when available) |
Create experiment analysis workflow parameters
Only applied options and parameter are listed.
| Differential Expression for RNA-Seq | |
| Technology | Small RNA |
| Normalization method | TMM |
| Filter on average expression for FDR correction | Yes |
| Create Heat Map for RNA-Seq | |
| Distance measure | Euclidean distance |
| Linkage criteria | Complete linkage |