-d, --destination <ClcServerObjectUrl> |
Destination file or folder on server. |
--ercc-concentration <ClcFileUrl> |
Thermo Fisher ERCC concentrations file containing the concentrations of the spike-ins in the various mixes. The files are available at the Thermo Fisher website. The file has the following headers: Re-sort ID, ERCC ID, subgroup, concentration in Mix 1 (attomoles/ul), concentration in Mix 2 (attomoles/ul), expected fold-change ratio, log2(Mix 1/Mix 2) |
--ercc-sequences <ClcFileUrl> |
Thermo Fisher ERCC annotation file containing the sequences and annotations. The file has the following headers: ERCC_ID, GenBank, 5prime_assay, 3prime_assay, Sequence |
--import-type <[TSV, ERCC]> |
Import settings (default: ERCC) |
--log <Boolean> |
Enable creation of algo log file. (default: true) |
--tsv-file <ClcFileUrl> |
Select a tab-separated file (.tsv, .txt) of spike-in data.The first column should contain the sequence name, the second column the nucleotide sequence, and the third and consecutive columns the concentrations of the spike-in measured in attomoles/?L. Multiple concentrations can be given to denote several mixes produced from the same sequences. Note Concentrations must not contain commas e.g. write 15000 rather than 15,000. |
