Restriction Based Cloning
The in silico cloning process in CLC Main Workbench begins with the Restriction Based Cloning tool:
Cloning (
)| Restriction Based Cloning (
)
This will open a dialog where you can select both the sequences containing the fragments you want to clone, as well as the one to be used as vector (figure 21.18).
Figure 21.18: Selecting the sequences containing the fragments you want to clone and the vector.
CLC Main Workbench will now create a sequence list of the selected fragments and vector sequences. For cloning work, open the sequence list and switch to the Cloning Editor () at the bottom of the view (figure 21.19).
Figure 21.19: Cloning editor view of the sequence list. Choose which sequence to display from the drop down menu.
If you later in the process need additional sequences, right-click anywhere on the empty white area of the view and choose to "Add Sequences".
Subsections