Demultiplex QIAseq UPX 3' reads

The UPX 3' RNA application of the Analyze QIAseq Panels guide offers a Demultiplex tool to be run before starting the Quantify QIAseq UPX 3' workflow (figure 6.1).

Image upxdemultiplex
Figure 6.1: The demultiplex workflow available from the Analyze QIAseq Panels guide.

Select the appropriate configuration depending on how the reads were generated - either NextSeq or MiSeq/HiSeq, and the appropriate plate size used for sequencing - before clicking Run. The workflow requires the reads sequenced with a QIAseq UPX 3' Transcriptome Kit or a QIAseq UPX 3' Targeted RNA Panel. Note that while it is possible only to use a subset of the wells in a plate (typically a row or a column in the 96 wells plate), the workflow will generate a demultiplexed sample even if there are only a few reads matching a barcode, thereby creating a larger number of samples than were sequenced.