Output from the Quantify QIAseq RNA Expression workflow

The Quantify QIAseq RNA Expression ready-to-use workflow produces expression tracks (Image rnaseqtrack_16_h_p) for each sample analyzed.

Expression tracks are displayed as tables listing genes included in the panel and several measures of their expression levels.

Expression tracks be further analyzed using statistical tools from the RNA-Seq Analysis folder of the workbench, such as PCA for RNA-Seq and Create Heat Map for RNA-Seq.

Why does the workflow not produce a read mapping?

The very short target regions in a QIAseq Targeted RNA Panel are not suited to downstream analyses that require a read mapping, such as variant calling. If a read mapping is desired, for example to investigate suspected off-target effects, we recommend using the Map Reads to Reference tool.