Perform QIAseq UPXome RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer)
The Perform QIAseq UPXome RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) template workflow supports a combined N6-T RT primer + ODT-RT primer protocol. All reads are used for gene expression analysis while the reads amplified by the N6-T RT primer are used for studying transcript expression and fusions.
The Perform QIAseq UPXome RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) workflow can be found here:
Template Workflows | Biomedical Workflows (
) | QIAseq Sample Analysis (
) | QIAseq RNA Workflows (
) | Perform QIAseq UPXome RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) (
)
Or run directly from the Analyze QIAseq Samples guide:
Template Workflows | Biomedical Workflows () | QIAseq Sample Analysis () | Analyze QIAseq Samples (
)
where it is available in the the UPXome RNA tab, under QIAseq UPXome Library Kit, and setting analysis to N6-T RT primer + ODT-RT primer.
The workflow is identical to the Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) workflow described in Perform QIAseq FastSelect RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) The only difference being that the Perform QIAseq UPXome RNA Expression and Fusion Calling (N6-T RT + ODT-RT primer) workflow starts by demultiplexing the input into samples that correspond to plate wells.
The workflow is run as described in Running the QIAseq UPXome workflows.
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