Output from the Identify Variants (TAS) workflow

The Identify Variants (TAS) tool produces six different types of output:

It is important that you do not delete any of the produced files individually as some of the outputs are linked to other outputs. If you would like to delete the outputs, please always delete all of them at the same time.

Please have first a look at the mapping report to see if the coverage is sufficient in regions of interest (e.g. > 30 ). Furthermore, please check that at least 90% of reads are mapped to the human reference sequence. In case of a targeted experiment, please also check that the majority of reads are mapping to the targeted region.

Afterwards please open the Genome Browser View file (see  15.43).

The Genome Browser View includes the track of identified variants in context to the human reference sequence, genes, transcripts, coding regions, targeted regions and mapped sequencing reads.

Image identify_variants_result1_tas
Figure 15.43: The Genome Browser View allows you to inspect the identified variants in the context of the human genome.

By double clicking on the variant track in the Genome Browser View, a table will be shown which includes information about all identified variants (see 15.44).

Image identify_variants_result2_tas
Figure 15.44: Genome Browser View with an open track table to inspect identified variants more closely in the context of the human genome.

In case you like to change the reference sequence used for mapping as well as the human genes, please use the "Data Management".